Review

psmd1 (a16420) (ABclonal Biotechnology)

Name: psmd1 (a16420)
Brand: ABclonal Biotechnology
Rating: 90 (1 reviews)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ABclonal Biotechnology psmd1 (a16420)
Psmd1 (A16420), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psmd1 (a16420)/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews

psmd1 (a16420) - by Bioz Stars, 2026-02

90/100 stars

Images

Image Search Results

PSMD1 and PSMD2 knockdown inhibits proliferation and induces cell apoptosis. a Cell proliferation was monitored with an MTT assay at the indicated time after treatment with PSMD1 and PSMD2 siRNAs in HepG2 cells. Data are presented as the mean ± SD (n = 3), *p < 0.05. b , c Flow cytometric analysis of the cell cycle distribution at 48 h post-transfection of the negative control (NC) or PSMD1 / PSMD2 siRNA in HepG2 cells. The percentages of each phase of the cell cycle (G0/G1, S, and G2/M) are shown. Data are presented as the mean ± SD (n = 3), *p < 0.05, **p < 0.01. d , e HepG2 cells were collected for the detection of apoptotic cells by flow cytometry, 48 h after transfection. In all panels, data are presented as the mean ± SEM of three independent assays. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (*p < 0.05; **p < 0.01) vs. the NC. f Relative mRNA expression of the cell cycle-related genes after transfection of siPSMD1/siPSMD2 and siNC. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01. g The mRNA expression levels of several apoptosis-related genes with siPSMD1/siPSMD2 and siNC in HepG2 cells. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01. h , i EdU staining after PSMD knockdown. The magnification is ×200. Results are shown as the mean ± SEM of three independent experiments. Independent sample t-tests were used to analysis the statistical differences between groups. *p < 0.05; **p < 0.01

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 knockdown inhibits proliferation and induces cell apoptosis. a Cell proliferation was monitored with an MTT assay at the indicated time after treatment with PSMD1 and PSMD2 siRNAs in HepG2 cells. Data are presented as the mean ± SD (n = 3), *p < 0.05. b , c Flow cytometric analysis of the cell cycle distribution at 48 h post-transfection of the negative control (NC) or PSMD1 / PSMD2 siRNA in HepG2 cells. The percentages of each phase of the cell cycle (G0/G1, S, and G2/M) are shown. Data are presented as the mean ± SD (n = 3), *p < 0.05, **p < 0.01. d , e HepG2 cells were collected for the detection of apoptotic cells by flow cytometry, 48 h after transfection. In all panels, data are presented as the mean ± SEM of three independent assays. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (*p < 0.05; **p < 0.01) vs. the NC. f Relative mRNA expression of the cell cycle-related genes after transfection of siPSMD1/siPSMD2 and siNC. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01. g The mRNA expression levels of several apoptosis-related genes with siPSMD1/siPSMD2 and siNC in HepG2 cells. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01. h , i EdU staining after PSMD knockdown. The magnification is ×200. Results are shown as the mean ± SEM of three independent experiments. Independent sample t-tests were used to analysis the statistical differences between groups. *p < 0.05; **p < 0.01

Article Snippet: The following antibodies were used: anti- PSMD1 (#A16420; rabbit polyclonal antibody; ABclonal; 1:1000 dilution), anti- PSMD2 (#14748-1-AP; rabbit polyclonal antibody; Proteintech; 1:1000 dilution), anti-ASK1 (#A6274; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti- p-ASK1 (#AP0394; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-AKT (#A11027; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p-AKT (#AP0140; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p38 (#A10832; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p-p38 (#AP0297; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-JNK1/2 (#A11119; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p-JNK1/2 (#AP0631; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-GAPDH (Servicebio, Wuhan, China; 1:2000 dilution), anti-Tubulin (Servicebio, Wuhan, China; 1:2000 dilution), HRP-labeled Goat Anti-Rabbit IgG (H + L) (#GB23303-1, Servicebio, Wuhan, China), and HRP-labeled Goat Anti-Mouse IgG (H + L) (#GB23301, Servicebio, Wuhan, China).

Techniques: Knockdown, MTT Assay, Transfection, Negative Control, Flow Cytometry, Expressing, Staining

PSMD1 and PSMD2 overexpression promotes proliferation and inhibits cell apoptosis. a Cell proliferation was monitored with the MTT assay at the indicated time after treatment with PSMD1 / PSMD2 overexpression in HepG2 cells. Data are presented as the mean ± SD (n = 3), *p < 0.05. b , c Flow cytometric analysis of the cell cycle distribution at 48 h post-transfection of overexpression plasmid in HepG2 cells. The percentages of each phase of the cell cycle (G0/G1, S, and G2/M) are shown. d , e HepG2 cells were collected for the detection of apoptotic cells by flow cytometry, 48 h after transfection. In all panels, data are presented as the mean ± SEM of three independent assays. f Relative mRNA expression of the cell cycle-related genes after transfection of the PSMD1 / PSMD2 plasmid. The statistical significance of differences between means was assessed using unpaired Student’s t-tests (*p < 0.05; **p < 0.01) vs. the NC. g The mRNA expression levels of several apoptosis-related genes with the overexpression plasmid in HepG2 cells. The statistical significance of differences between means was assessed using unpaired Student’s t-tests (*p < 0.05; **p < 0.01) vs. the NC. h , i EdU staining after PSMD1 / PSMD2 overexpression. The magnification is 200×. Results are shown as the mean ± SEM of three independent experiments. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 overexpression promotes proliferation and inhibits cell apoptosis. a Cell proliferation was monitored with the MTT assay at the indicated time after treatment with PSMD1 / PSMD2 overexpression in HepG2 cells. Data are presented as the mean ± SD (n = 3), *p < 0.05. b , c Flow cytometric analysis of the cell cycle distribution at 48 h post-transfection of overexpression plasmid in HepG2 cells. The percentages of each phase of the cell cycle (G0/G1, S, and G2/M) are shown. d , e HepG2 cells were collected for the detection of apoptotic cells by flow cytometry, 48 h after transfection. In all panels, data are presented as the mean ± SEM of three independent assays. f Relative mRNA expression of the cell cycle-related genes after transfection of the PSMD1 / PSMD2 plasmid. The statistical significance of differences between means was assessed using unpaired Student’s t-tests (*p < 0.05; **p < 0.01) vs. the NC. g The mRNA expression levels of several apoptosis-related genes with the overexpression plasmid in HepG2 cells. The statistical significance of differences between means was assessed using unpaired Student’s t-tests (*p < 0.05; **p < 0.01) vs. the NC. h , i EdU staining after PSMD1 / PSMD2 overexpression. The magnification is 200×. Results are shown as the mean ± SEM of three independent experiments. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01

Techniques: Over Expression, MTT Assay, Transfection, Plasmid Preparation, Flow Cytometry, Expressing, Staining

PSMD1 and PSMD2 overexpression regulates the number and size of cellular lipid droplets. a Detection of the cellular LDs marked by BODIPY493/503 through a laser scanning confocal microscope. The HepG2 cells were seeded on the slide in a 24-well plate. Then, the cells were transfected with PSMD1/PSMD2 or NC overexpression plasmid for 48 h. Subsequently, the cells were treated with 200 μM oleic acid for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI for observation by microscope. b , c The number of cellular LDs from different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 20; *p < 0.05; **p < 0.01) vs. NC. d , e The size of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 10; *p < 0.05; **p < 0.01) vs. NC

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 overexpression regulates the number and size of cellular lipid droplets. a Detection of the cellular LDs marked by BODIPY493/503 through a laser scanning confocal microscope. The HepG2 cells were seeded on the slide in a 24-well plate. Then, the cells were transfected with PSMD1/PSMD2 or NC overexpression plasmid for 48 h. Subsequently, the cells were treated with 200 μM oleic acid for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI for observation by microscope. b , c The number of cellular LDs from different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 20; *p < 0.05; **p < 0.01) vs. NC. d , e The size of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 10; *p < 0.05; **p < 0.01) vs. NC

Techniques: Over Expression, Microscopy, Transfection, Plasmid Preparation, Staining, Software

PSMD1 and PSMD2 regulate the expression level of fatty acids (FAs) and lipid synthesis-related genes. a Interference efficiency detection by qRT-PCR. b The expression level of fatty acid synthesis-related genes was detected by qRT-PCR. c The expression level of lipid synthesis-related genes was detected by qRT-PCR. d Overexpression efficiency detection by qRT-PCR. e The expression level of fatty acid synthesis-related genes was detected by qRT-PCR. f The expression level of lipid synthesis-related genes was detected by qRT-PCR. g The ASK1-p38-JNK and AKT signaling in groups of interfered cells and control cells was detected by Western Blot experiments. TUBULIN and GAPDH were the reference proteins. h Grey value analysis of g . ImageJ software was used for this analysis, according to the instructions. i The ASK1-p38-JNK and AKT signaling in the overexpression cell group and control cells was detected by Western Blot experiments. TUBULIN and GAPDH were the reference proteins. j Grey value analysis of i . ImageJ software was used for this analysis, according to the instructions. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 3; *p < 0.05; **p < 0.01) vs. NC

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 regulate the expression level of fatty acids (FAs) and lipid synthesis-related genes. a Interference efficiency detection by qRT-PCR. b The expression level of fatty acid synthesis-related genes was detected by qRT-PCR. c The expression level of lipid synthesis-related genes was detected by qRT-PCR. d Overexpression efficiency detection by qRT-PCR. e The expression level of fatty acid synthesis-related genes was detected by qRT-PCR. f The expression level of lipid synthesis-related genes was detected by qRT-PCR. g The ASK1-p38-JNK and AKT signaling in groups of interfered cells and control cells was detected by Western Blot experiments. TUBULIN and GAPDH were the reference proteins. h Grey value analysis of g . ImageJ software was used for this analysis, according to the instructions. i The ASK1-p38-JNK and AKT signaling in the overexpression cell group and control cells was detected by Western Blot experiments. TUBULIN and GAPDH were the reference proteins. j Grey value analysis of i . ImageJ software was used for this analysis, according to the instructions. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 3; *p < 0.05; **p < 0.01) vs. NC

Techniques: Expressing, Quantitative RT-PCR, Over Expression, Control, Western Blot, Software

PSMD1 and PSMD2 regulate cellular lipid metabolism. HepG2 cells were seeded in the 6-well plate. Then, the cells were transfected with PSMD1 / PSMD2 siRNAs or SREBF1 / PPARγ overexpression vectors or control siRNA or a control overexpression vector. a , d Cell proliferation capacity was monitored by the EdU assay. b , e Statistical analysis was performed on the EdU assay. The magnification is 200×. Results are shown as the mean ± SEM of three independent experiments. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01. c , f Relative mRNA expression of the cell-cycle-related genes. Independent sample t-tests were used to analysis the statistical differences between groups (n = 3), *p < 0.05; **p < 0.01

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 regulate cellular lipid metabolism. HepG2 cells were seeded in the 6-well plate. Then, the cells were transfected with PSMD1 / PSMD2 siRNAs or SREBF1 / PPARγ overexpression vectors or control siRNA or a control overexpression vector. a , d Cell proliferation capacity was monitored by the EdU assay. b , e Statistical analysis was performed on the EdU assay. The magnification is 200×. Results are shown as the mean ± SEM of three independent experiments. Independent sample t-tests were used to analyze the statistical differences between groups. *p < 0.05; **p < 0.01. c , f Relative mRNA expression of the cell-cycle-related genes. Independent sample t-tests were used to analysis the statistical differences between groups (n = 3), *p < 0.05; **p < 0.01

Techniques: Transfection, Over Expression, Control, Plasmid Preparation, EdU Assay, Expressing

PSMD1 and PSMD2 localize to the surface of lipid droplets. Fluorescence expression vectors were constructed, including PSMD1 -mCherry, PSMD2 -mCherry, PSMD1 -EGFP, PLIN2-EGFP, and Livedrop-EGFP. a PSMD1 -mCherry or PSMD2 -mCherry was transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). b PSMD1 -EGFP and PSMD2 -mCherry were co-transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). The fluorescence plot was analyzed by ImageJ software. c , d The cells were transfected with PSMD1 -mCherry or PSMD2 -mCherry for 48 h, and then the cells were treated with 200 μM oleic acid (OA) for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). e , f The cells were co-transfected with PSMD1 -mCherry or PSMD2 -mCherry and PLIN2-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). g , h The cells were co-transfected with PSMD1 -mCherry or PSMD2 -mCherry and Livedrop-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm)

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 localize to the surface of lipid droplets. Fluorescence expression vectors were constructed, including PSMD1 -mCherry, PSMD2 -mCherry, PSMD1 -EGFP, PLIN2-EGFP, and Livedrop-EGFP. a PSMD1 -mCherry or PSMD2 -mCherry was transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). b PSMD1 -EGFP and PSMD2 -mCherry were co-transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). The fluorescence plot was analyzed by ImageJ software. c , d The cells were transfected with PSMD1 -mCherry or PSMD2 -mCherry for 48 h, and then the cells were treated with 200 μM oleic acid (OA) for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). e , f The cells were co-transfected with PSMD1 -mCherry or PSMD2 -mCherry and PLIN2-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). g , h The cells were co-transfected with PSMD1 -mCherry or PSMD2 -mCherry and Livedrop-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm)

Techniques: Fluorescence, Expressing, Construct, Transfection, Staining, Microscopy, Software

PSMD1 and PSMD2 show higher expression in liver hepatocellular carcinoma (LIHC) and are associated with a poor prognosis. Gene expression analysis and survival analysis were performed by the GEPIA (gene expression profiling interactive analysis) database ( http://gepia.cancer-pku.cn/ ). a , b Box plot of expression of PSMD1 and PSMD2 in LIHC. c , d The survival analysis of PSMD1 and PSMD2 in LIHC

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 show higher expression in liver hepatocellular carcinoma (LIHC) and are associated with a poor prognosis. Gene expression analysis and survival analysis were performed by the GEPIA (gene expression profiling interactive analysis) database ( http://gepia.cancer-pku.cn/ ). a , b Box plot of expression of PSMD1 and PSMD2 in LIHC. c , d The survival analysis of PSMD1 and PSMD2 in LIHC

Techniques: Expressing, Gene Expression

PSMD1 and PSMD2 knockdown regulates the number and size of cellular lipid droplets. a Detection of the cellular LDs marked by BODIPY493/503 through a laser scanning confocal microscope. The HepG2 cells were seeded on the slide in a 24-well plate. Then, the cells were transfected with PSMD1/PSMD2 or NC siRNAs for 48 h. Subsequently, the cells were treated with 200 μM oleic acid for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI for observation by microscope. b , c The number of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 20; *p < 0.05; **p < 0.01) vs. NC. d , e The count of the size of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. Statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 10; *p < 0.05; **p < 0.01) vs. NC

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: PSMD1 and PSMD2 knockdown regulates the number and size of cellular lipid droplets. a Detection of the cellular LDs marked by BODIPY493/503 through a laser scanning confocal microscope. The HepG2 cells were seeded on the slide in a 24-well plate. Then, the cells were transfected with PSMD1/PSMD2 or NC siRNAs for 48 h. Subsequently, the cells were treated with 200 μM oleic acid for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI for observation by microscope. b , c The number of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 20; *p < 0.05; **p < 0.01) vs. NC. d , e The count of the size of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. Statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 10; *p < 0.05; **p < 0.01) vs. NC

Techniques: Knockdown, Microscopy, Transfection, Staining, Software

Overview of the regulatory mechanism of PSMD1 and PSMD2 that regulates tumor cell proliferation. Briefly, PSMD1 and PSMD2 regulate the phosphorylation of ASK1-p38-JNK and AKT signaling. Then, the expression level of SREBF1 and PPARγ is regulated. Subsequently, these two important transcriptional factors regulate the expression levels of FAs and lipid synthesis-related genes such as FASN, SCD1, ASCL, DGAT and PLIN . The upregulation of these genes induces more lipid droplet (LD) formation, whereas LDs can provide membrane components and energy for cell proliferation. Therefore, PSMD1 and PSMD2 regulate cell proliferation via modulating cellular lipid metabolism

Journal: BMC Molecular Biology

Article Title: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

doi: 10.1186/s12867-019-0141-z

Figure Lengend Snippet: Overview of the regulatory mechanism of PSMD1 and PSMD2 that regulates tumor cell proliferation. Briefly, PSMD1 and PSMD2 regulate the phosphorylation of ASK1-p38-JNK and AKT signaling. Then, the expression level of SREBF1 and PPARγ is regulated. Subsequently, these two important transcriptional factors regulate the expression levels of FAs and lipid synthesis-related genes such as FASN, SCD1, ASCL, DGAT and PLIN . The upregulation of these genes induces more lipid droplet (LD) formation, whereas LDs can provide membrane components and energy for cell proliferation. Therefore, PSMD1 and PSMD2 regulate cell proliferation via modulating cellular lipid metabolism

Techniques: Phospho-proteomics, Expressing, Membrane

Review

Structured Review

Images

Similar Products

Image Search Results